The frequency of populations must be displayed on the plot or in a table The number of events in a plot must be displayed in the plot or figure legend. Med.Īxis on plots must have the reagent and fluorescence labelled (ex. This will be done in an excel template that will be available from ISAC or J. Once a comprehensive list is created, I'll post them for the group to see.Īuthor must list the instrument(s) used, the laser(s) used for excitation, and the filter(s) used for emission. Here are a few examples from the guidelines. Mario Roederer, shared the conclusions of the committee this week at the ISAC Congress. A member of the Data Standards Committee, Dr. Being the premier flow cytometry authority, ISAC has taken it upon itself to lay down some guidelines to assist authors in properly presenting the necessary information to describe their flow cytometric data. It's not that the data is bad, or doesn't support the authors conclusions, it's just that the figure is annotated so poorly, and the methods written so vaguely that it's nearly impossible for anyone to try and repeat complicated analyses. What the cytometry field has been noticing for quite a while is the amount of publications in high-end journals with really poor flow cytometry figures. anytime soon? Well then you'll want to pay attention to the new guidelines generated by the Data Presentation Standards Committee and adopted by J.
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